Oxalate decarboxylase activity was present in liquid culture medium and myc
elium of Agaricus bisporus. Enzyme activity in the mycelium peaked at two-w
eekly intervals after primary growth phase and into secondary metabolism wi
th activity peaks in the medium occurring 7 d later than in the mycelium. T
he enzyme was partially purified and had two isozymes with pls 3.0 and 3.4.
Characterization of the protein by SDS-PAGE and Western blotting against a
polyclonal antibody raised to oxalate decarboxylase from Collybia velutipe
s showed a major protein band of 64 kDa. Oxalate decarboxylase was also det
ected in the fruit body up to the cup stage of development. The pH optimum
for the enzyme was 3.6 and temperature optimum was 35 degrees C.