THE USE OF GASEOUS AMMONIA FOR THE DEPROTECTION AND CLEAVAGE STEPS DURING THE SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES, AND ANALOGS

Authors
IYER RP YU D XIE J ZHOU W AGRAWAL S
Citation
Rp. Iyer et al., THE USE OF GASEOUS AMMONIA FOR THE DEPROTECTION AND CLEAVAGE STEPS DURING THE SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES, AND ANALOGS, Bioorganic & medicinal chemistry letters, 7(11), 1997, pp. 1443-1448
Citations number
18
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
7
Issue
11
Year of publication
1997
Pages
1443 - 1448
Database
ISI
SICI code
0960-894X(1997)7:11<1443:TUOGAF>2.0.ZU;2-J
Abstract
The use of NH3 gas under pressure offers an efficient alternative to h ot aqueous ammonium hydroxide for the deprotection, and cleavage steps during the large-scale synthesis of oligonucleotides, and their analo gs prepared using N-pent-4-enoyl (PNT) nucleoside phosphoramidites and H-phosphonates. (C) 1997 Elsevier Science Ltd.