T. Bahar et A. Tuncel, Immobilization of alpha-chymotrypsin onto newly produced poly(hydroxypropyl methacrylate-co-methacrylic acid) hydrogel beads, REACT FUNCT, 44(1), 2000, pp. 71-78
In this study, recently produced poly(hydroxypropyl methacrylate-co-methacr
ylic acid) gel beads were tried as a carrier matrix for the immobilization
of ol-chymotrypsin (CT). A water soluble carbodiimide (WSC) activation proc
edure was applied for the covalent binding of CT via carboxyl groups of the
selected carrier. The immobilization conditions (i.e., WSC and enzyme conc
entrations and immobilization pH) providing maximum retained activity after
the immobilization were determined. The kinetic behaviour of immobilized a
-chymotrypsin was investigated in a basket type batch reactor. The highest
activity was observed at a pH of 7.8 for either free or immobilized enzyme.
The temperature stability was significantly improved by the immobilization
. The K-M value of immobilized CT was 140 mu M while the free enzyme K-M wa
s 63 mu M No significant loss was observed in the activity of immobilized e
nzyme stored in an aqueous buffer solution at +4 degrees C for 8 months. (C
) 2000 Elsevier Science B.V. All rights reserved.