RAPDS AS MOLECULAR MARKERS FOR THE DETECTION OF AEGILOPS MARKGRAFII CHROMATIN IN ADDITION AND EUPLOID INTROGRESSION LINES OF HEXAPLOID WHEAT

Aegilops markgrafii contains resistance genes to powdery mildew, leaf rust and stripe rust: and also has high crude protein and lysine conte nts, which can be useful for wheat improvement, These important traits are localized on different chromosomes. Disomic Triticum aestivum-Ae. markgrafii addition lines and euploid introgression lines showing lea f-rust and powdery mildew resistance were screened with RAPDs to detec t chromosome-specific markers which can accelerate the breeding proces s, RAPD markers for all six available disomic addition lines were obta ined. The additional chromosomes B, C, D, E, F and G were identified b y three, three, three, two, one and seven primers, respectively. All t hree chromosome-B-specific RAPD markers demonstrated the presence of a lien chromatin in the leaf-rust-resistant 42-chromosome introgression lines as well as in the segregating progeny. The three chromosome-C-id entifying primers also demonstrated the presence of that chromosome in powdery mildew-resistant euploid introgression lines. The substitutio n lines (5A)5C and (5D)5C with different genetic backgrounds for both parents, in comparison to the lines mentioned above, showed the chromo some C-specific band with only two of the three primers. The chromosom e F-specific primer and a primer evident on all the Ae. markgrafii chr omosomes analysed did not generate the expected fragments on the chrom osome F-del addition line, indicating that the markers are located on the deleted part of chromosome F.