Td. Mchugh et al., IS6110 HOMOLOGS ARE PRESENT IN MULTIPLE COPIES IN MYCOBACTERIA OTHER THAN TUBERCULOSIS-CAUSING MYCOBACTERIA, Journal of clinical microbiology, 35(7), 1997, pp. 1769-1771
We have previously demonstrated homology between a 181-bp fragment of
IS6110 and DNA from mycobacteria other than tuberculosis-causing mycob
acteria (MOTT). Genomic DNA from 14 strains of MOTT was digested with
PvuII and was hybridized,vith a probe derived from the 181-bp fragment
and the INS1/INS2 international standard probe at high stringency, Mu
ltiple banding patterns were obtained from isolates of M. avium-M. int
racellulare, M. fortuitum, M. kansasii, and M. malmoense. Differences
in the banding patterns between and within species were obtained, This
suggests that mycobacteria possess a family of IS3-like elements. The
species of isolates suspected of being M. tuberculosis must be carefu
lly determined before IS6110 restriction fragment length polymorphism
analysis, and caution must be used in designing and evaluating diagnos
tic PCR tests based on this element.