COMPARISON OF MADIN-DARBY CANINE KIDNEY-CELLS (MDCK) WITH A GREEN MONKEY CONTINUOUS CELL-LINE (VERO) AND HUMAN LUNG EMBRYONATED CELLS (MRC-5) IN THE ISOLATION OF INFLUENZA-A VIRUS FROM NASOPHARYNGEAL ASPIRATESBY SHELL VIAL CULTURE

Authors
REINA J FERNANDEZBACA V BLANCO I MUNAR M
Citation
J. Reina et al., COMPARISON OF MADIN-DARBY CANINE KIDNEY-CELLS (MDCK) WITH A GREEN MONKEY CONTINUOUS CELL-LINE (VERO) AND HUMAN LUNG EMBRYONATED CELLS (MRC-5) IN THE ISOLATION OF INFLUENZA-A VIRUS FROM NASOPHARYNGEAL ASPIRATESBY SHELL VIAL CULTURE, Journal of clinical microbiology, 35(7), 1997, pp. 1900-1901
Citations number
13
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
7
Year of publication
1997
Pages
1900 - 1901
Database
ISI
SICI code
0095-1137(1997)35:7<1900:COMCK(>2.0.ZU;2-6
Abstract
We report a comparative study of the MDCK, Vero, and MRC-5 cell lines in the isolation of the influenza A (IA) virus. We studied 746 samples in which 63 IA viruses were isolated. The MDCK line displayed 100% se nsitivity, the Vero line displayed 71.4%, and the MRC-5 displayed 57.1 %. The MDCK line showed a statistically significant difference with re spect to the Vero line (P = 0.001) and the MRC-5 line (P = 0.001), The quantitative sensitivity analysis showed the MDCK line to be superior to the other lines. It stems that the MDCK line is still one of the m ost recommendable for the isolation of the IA virus from respiratory s amples.