Polypeptide synthesis using an expressed peptide as a building block via the thioester method

Authors
Kawakami, T Hasegawa, K Teruya, K Akaji, K Horiuchi, M Inagaki, F Kurihara, Y Uesugi, S Aimoto, S
Citation
T. Kawakami et al., Polypeptide synthesis using an expressed peptide as a building block via the thioester method, TETRAHEDR L, 41(15), 2000, pp. 2625-2628
Citations number
9
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
41
Issue
15
Year of publication
2000
Pages
2625 - 2628
Database
ISI
SICI code
0040-4039(20000408)41:15<2625:PSUAEP>2.0.ZU;2-E
Abstract
An expressed peptide was proven to be useful as a building block in the syn thesis of a polypeptide via the thioester method. A partially protected pep tide segment, [Lys(Boc)(15,25,31,48,57,68,80,95)]-Max(14-101), was prepared via transamination of an N-terminal amino group from p21Max(13-101), which was obtained by expression using Escherichia coli. This peptide was utiliz ed in the synthesis of [Ser(PO3H2)(2,11)]-p21Max(1-101) via condensation wi th the peptide thioester, Boc-[Ser(PO3H2)(2,11)]-p21Max(1-13)-SCH2CH2CO-Leu -OH. (C) 2000 Elsevier Science Ltd. All rights reserved.