PriA-directed replication fork restart in Escherichia coli

The encounter of a replication fork with either a damaged DNA template, a n ick in the template strand or a 'frozen' protein-DNA complex can stall the replisome and cause it to fall apart. Such an event generates a requirement for replication fork restart if the cell is going to survive. Recent evide nce shows that replication fork restart is effected by the action of the re combination proteins generating a substrate for PriA-directed replication f ork assembly.