VASCULAR EXPRESSION OF ENDOTHELIAL ANTIGEN PAL-E INDICATES ABSENCE OFBLOOD-OCULAR BARRIERS IN THE NORMAL EYE

The endothelium-specific antigen PAL-E is expressed in capillaries and veins throughout the body with the exception of the brain, where the antigen is absent from anatomical sites with a patent blood-brain barr ier. In this study we determined vascular endothelial staining for PAL -E in the normal eye in relation to the ocular blood-tissue barriers. Immunohistochemical staining of frozen tissue sections of eyes from 22 cornea donors and a number of normal animal autopsy eyes was performe d for the PAL-E antigen and the blood-brain barrier marker glucose tra nsporter 1. In normal human and animal eyes, endothelial PAL-E stainin g was absent from the microvasculature in iris, ciliary muscle, optic nerve and retina. In a few normal human eyes, some weakly stained capi llaries were observed in the retina and nerve fiber layer, mostly in t he peripapillary area. Marked staining of capillaries and venules with PAL-E was observed in the conjunctiva, episclera, sclera, ciliary pro cesses, choriocapillaris and optic nerve head, In general, the endothe lial antigen PAL-E is absent from microvessels involved in the blood-o cular and the blood-retinal barriers. PAL-E may therefore be a useful marker to identify pathological breakdown of blood-ocular barriers.