A study was performed to detect the presence of polycyclic aromatic hy
drocarbons (PAHs) in bovine eyes. Twenty fresh bovine eyes were used t
o detect the presence of PAHs in lens, vitreous and aqueous by HPLC an
d spectrofluorometer. In lenticular tissue the mean amount of PAHs was
0.0271 mu g/g and the mean level of PAH in each lens was 0.059 mu g.
Five types of PAHs (pyrene, fluoranthene, triphenylene, 1.2-benzanthra
cene and chrysene) were found in the lenses but none in vitreous and a
queous. These data indicate that PAHs are present only in the lens of
the bovine eye. The source of these substances in mammalian clear lens
is unclear.