Direct correlation of glutathione and ascorbate and their dependence on age and season in human lymphocytes

Background: Endogenous reactive oxygen species appear to contribute to agin g and cancer and dietary antioxidants, present in fruit and vegetables, cou nteract these effects. Objective: The objective was to examine the association between intracellul ar glutathione, ascorbate (vitamin C), and oc-tocopherol (vitamin E) in hum an lymphocytes. Design: The study group consisted of 240 healthy nonsmoking volunteers with an approximately equal number of male and female subjects subdivided into 3 age groups: 18-39, 40-59, and greater than or equal to 60 y). Glutathione , glutathione disulfide, ascorbate, and alpha-tocopherol were measured in l ymphocytes by HPLC. Results: The average concentration of antioxidants in lymphocytes was 27 +/ - 8 nmol/mg protein for glutathione, 21 +/- 8 nmol/mg protein for ascorbate , and 0.4 +/- 0.2 nmol/mg protein for alpha-tocopherol. There was a strong positive correlation between glutathione and ascorbate (r = 0.62, P < 0.001 ). No correlation was observed for glutathione and ascorbate with alpha-toc opherol. The concentration of glutathione in lymphocytes was inversely corr elated with age (r = -0.19, P < 0.01), as was that of ascorbate (r = -0.22, P < 0.01), with 10-20% lower values in elderly than in young and elderly s ubjects. The concentrations of glutathione in lymphocytes were as much as 2 5% higher and those of ascorbate were as much as 38% higher during the summ er than during the winter. The seasonal variation of ascorbate in lymphocyt es was described by a linear function for age and a periodic sine function for season. Conclusion: Glutathione and ascorbate are directly correlated in human lymp hocytes.