Use of capillary electrophoresis and indirect detection to quantitate in-capillary enzyme-catalyzed microreactions

The use of capillary electrophoresis and indirect detection to quantify rea ction products of in-capillary enzyme-catalyzed microreactions is described . Migrating in a capillary under conditions of electrophoresis, plugs of en zyme and substrate are injected and allowed to react. Capillary electrophor esis is subsequently used to measure the extent of reaction. This technique is demonstrated using two model systems: the conversion of fructose-1,6-bi sphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate by fructose-biphosphate aldolase (ALD, EC 4.1.2.13), and the conversion of fru ctose-l,6-bisphosphate to fructose-6-phosphate by fructose-1,6-bisphospatas e (FBPase, EC 3.1.3.11). These procedures expand the use of the capillary a s a microreactor and offer a new approach to analyzing enzyme-mediated reac tions.