Direct and indirect effects of P2O5 glass reinforced-hydroxyapatite composites on the growth and function of osteoblast-like cells

Citation
Ma. Lopes et al., Direct and indirect effects of P2O5 glass reinforced-hydroxyapatite composites on the growth and function of osteoblast-like cells, BIOMATERIAL, 21(11), 2000, pp. 1165-1172
Citations number
30
Categorie Soggetti
Multidisciplinary
Journal title
BIOMATERIALS
ISSN journal
01429612 → ACNP
Volume
21
Issue
11
Year of publication
2000
Pages
1165 - 1172
Database
ISI
SICI code
0142-9612(200006)21:11<1165:DAIEOP>2.0.ZU;2-B
Abstract
Human osteoblast-like cells were plated on hydroxyapatite and P2O5-glass re inforced hydroxyapatite composite discs. They were also cultured in the pre sence of media obtained by incubating the discs in the absence of cells. Th e effects of these direct and indirect interactions were examined by measur ing cell proliferation and the expression of certain key extracellular matr ix antigens. One composite was found to initially delay cell growth, while the extract of a different composite appeared to down-regulate DNA synthesi s. Flow cytometry analysis showed that growth directly on the discs had lit tle effect on collagen type I, but reduced fibronectin and osteocalcin leve ls. The extracts of the materials generally had less effect, although one e xtract obtained from the glass-reinforced hydroxyapatite significantly down -regulated fibronectin. These in vitro studies thus suggest that there were only few differences overall in the growth of the cells directly on the gl ass-reinforced compared with the hydroxyapatite discs and also only relativ ely small effects of the extracts on the cells. However, the flow cytometry results suggest that both the materials and the extracts may have a potent ially important influence on connective tissue production, and that these e ffects are both material- and antigen-specific. (C) 2000 Elsevier Science L td. All rights reserved.