To study the biology of basal laminae in the developing nervous system the
protein composition of the embryonic retinal basal lamina was investigated,
the site of synthesis of its proteins in the eye was determined, and basal
lamina assembly was studied in vivo in two assay systems. Laminin, nidogen
, agrin, collagen IV, and XVIII are major constituents of the retinal basal
lamina. However, only agrin is synthesized by the retina, whereas the othe
r matrix constituents originate from cells of the ciliary body, the lens, o
r the optic disc. The synthesis from extraretinal tissues infers that the r
etinal basal lamina proteins must be shed from their tissues of origin into
the vitreous body and from there bind to receptor proteins provided by the
retinal neuroepithelium. The fact that all proteins typical for the retina
l basal lamina are abundant in the vitreous body and a new basal lamina is
only formed when the vitreous body was directly adjacent to the retina is c
onsistent with the contention of the vitreous body having a function in ret
inal basal lamina formation. Basal lamina assembly was also studied after d
isrupting the retinal basal lamina by intraocular injection of collagenase.
The basal lamina regenerated after chasing the collagenase with Matrigel,
which served as a collagenase inhibitor. The basal lamina was reconstituted
within 6 h. However, the regenerated basal lamina was located deeper in th
e retina than normal by reconstituting along the retracted neuroepithelial
endfeet demonstrating that these endfeet are the preferred site of basal la
mina assembly. (C) 2000 Academic Press.