Levels of methyleugenol in a subset of adults in the general US populationas determined by high resolution mass spectrometry

Citation
Db. Barr et al., Levels of methyleugenol in a subset of adults in the general US populationas determined by high resolution mass spectrometry, ENVIR H PER, 108(4), 2000, pp. 323-328
Citations number
12
Categorie Soggetti
Environment/Ecology,"Pharmacology & Toxicology
Journal title
ENVIRONMENTAL HEALTH PERSPECTIVES
ISSN journal
00916765 → ACNP
Volume
108
Issue
4
Year of publication
2000
Pages
323 - 328
Database
ISI
SICI code
0091-6765(200004)108:4<323:LOMIAS>2.0.ZU;2-Q
Abstract
We developed a sensitive and accurate analytical method for quantifying met hyleugenol (ME) in human serum. Our method uses a simple solid-phase extrac tion followed by a highly specific analysis using isotope dilution gas chro marography-high resolution mass spectrometry. Our method is very accurate; its limit of detection is 3.1 pg/g and its average coefficient of variation is 14% over a 200-pg/g range. We applied this method to measure serum ME c oncentrations in adults in the general U.S. population. ME was detected in 98% of our samples, with a mean ME concentration of 24 pg/g (range < 3.1-39 0 pg/g). Lipid adjustment of the data did not alter the distribution. Bivar iate and multivariate analyses using selected demographic variables showed only marginal relationships between race/ethnicity and sex/fasting status w ith serum ME concentrations. Although no demographic variable was a good pr edictor of ME exposure or dose, our data indicate prevalent exposure of U.S . adults to ME. Detailed pharmacokinetic studies are required to determine the relationship between ME intake and human serum ME concentrations.