Two RAPD markers linked to a major fertility restorer gene in pepper

Both major and minor genes control the restoring of fertility in the cytopl asmic male-sterility system in pepper (Capsicum annuum L.). Bulked segregan t analysis (BSA) was applied to identify molecular markers linked to a majo r restorer gene (Rf) using the F-2 population of Niujiaojiao No.21 (rfrf)/X iangtanwan (RfRf). Two random amplified polymorphic DNA (RAPD) markers link ed to this allele were detected with 520 decamer primers with arbitrary seq uences. OP13(1400) is a tightly linked marker with a genetic distance of 0. 37 cm. OW19(800) is on the opposite side with a distance of 8.12 cm. Both m arkers were repeatable and easy to score. A panel of genotypes, including 1 3 elite inbred lines with different fertility restoring ability, were assay ed for the presence of OP13(1400) and OW19(800). The markers are absent in all sweet pepper lines, indicating that they will be most helpful for trans ferring Rf into sweet pepper lines. With the aid of these markers, the size of the backcross population for testcrosses can be minimized. Furthermore, these markers will be useful in genetic analysis of the minor genes.