M. Karas et al., Nano-electrospray ionization mass spectrometry: addressing analytical problems beyond routine, FRESEN J AN, 366(6-7), 2000, pp. 669-676
The advent of nano-electrospray ionization (nano-ESI) has considerably exte
nded the usability of ESI in the analytical mass spectrometric laboratory.
One of the remarkable features of nano-ESI is its extremely low sample cons
umption. Only a few microliters of analyte solution (10(-5)-10(-8) M) are s
ufficient for molecular weight determination and structural investigations
by MS/MS. But nano-ESI is more than just a minimized-flow ESI; the low solv
ent flow rate also affects the mechanism of ion formation. As a consequence
, the area of ESI-MS applications is significantly enhanced. Oligosaccharid
es, glycosides as well as glycoproteins can be analyzed more easily than wi
th normal ion spray. The same holds for the analysis of non-covalent comple
xes sprayed directly from aqueous solutions.