Evidence that hyaluronidase is not involved in tissue invasion of the protozoan parasite Entamoeba histolytica.

As previous reports suggested that a hyaluronidase is involved in tissue in vasion of Entamoeba histolytica, we searched for such an activity in tropho zoite extracts. A hyaluronidase activity was not detectable in long-term cu ltures or in amoebae freshly passaged through a gerbil liver, as evidenced by four different techniques.