Application of staphylococcal enterotoxin B on normal and atopic skin induces up-regulation of T cells by a superantigen-mediated mechanism

Background: The skin of patients with inflammatory skin diseases such as at opic dermatitis is frequently colonized with Staphylococcus aureus. Coloniz ation with S aureus has been reported to exacerbate atopic dermatitis. Rece nt studies have demonstrated that S aureus isolated from the skin of patien ts with atopic dermatitis releases bacterial toxins that act as superantige ns. We have previously applied the staphylococcal superantigen staphylococc al enterotoxin B (SEB) on intact human skin and found that the application led to induction of dermatitis. Objective: The purpose of the study was to determine whether superantigen-i nduced dermatitis is primarily due to a T cell-superantigen-mediated reacti on or represents nonspecific cytokine-driven inflammation. Methods: We applied SEE, vehicle, and sodium lauryl sulfate on normal skin in healthy (n = 6) and atopic subjects (n = 6) and biopsy specimens were ta ken from all treated areas. The biopsy specimens from all subjects and peri pheral blood from the atopic subjects were analyzed for the T-cell receptor (TCR) V beta repertoire with mAbs against TCR V beta 2, 3, 8.1, 12, 14, an d 17. Results: From all subjects, both healthy and patients with atopic dermatiti s, skin biopsy specimens from SEE-treated areas demonstrated selective accu mulation of T cells expressing SEE-reactive TCR V beta 12 and 17 (P < .05). This selective up-regulation was not found in the sodium lauryl sulfate-tr eated areas. Conclusion: Our data strongly support that superantigen-induced T-cell acti vation is involved in the dermatitis seen after experimental application of SEE on intact skin.