Simultaneous multiwavelength detection based on surface plasmon resonance technique

The present article covers a navel surface plasmon resonance (SPR) instrume nt based on simultaneous multiwavelength detection. The sensing principle o f a traditional SPR device is based on fixing a discrete excitation wavelen gth and modulating the angle of incident light. The SPR-reflected spectra a re shown in terms of reflected light intensity versus angle of incident lig ht. The instrument presented here is designed on the basis of fixing the an gle of incident light and measuring the change of resonant wavelength in th e wavelength range of 400-800 nm. The SPR spectra are shown in terms of ref lected light intensify versus wavelength of the incident light. The intensi ty of the reflected light is the minimum at the resonant wavelength. With t he aid of the device, some chemical sensors and immunosensors, such as the thiolate chemical sensor, the fibrin immunosensor, and the albumin immunose nsor were developed. The kinetic processes of formation of various sensing monolayers were studied in real time through observing the change of resona nt wavelength. (C) 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:104-107, 2000.