DNA damage analysed with comet assay and aberration frequency analysed with classical cytogenetics and fish after in vitro 252-Californium neutron irradiations of human lymphocytes
D. Florjan et al., DNA damage analysed with comet assay and aberration frequency analysed with classical cytogenetics and fish after in vitro 252-Californium neutron irradiations of human lymphocytes, NEOPLASMA, 46, 1999, pp. 88-89
The aim of this study was to evaluate the effectiveness of DNA and chromoso
mal damage induction in human lymphocytes exposed in vitro to neutrons from
a Californium-252 isotopic source at very low dose rate, i.e. 0.001 Gy/min
, at a dose range of 0 - 0.8 Gy (chronic radiation). SCGE assay, classical
cytogenetics and FISH with whole painting probes for chromosomes 1 and 4, a
nd pancentromeric probe were applied to investigate this problem. There is
an increase in DNA damage and aberration frequency with dose of radiation.
The analysis of the results revealed a linear dose-dependent response in th
e whole investigated dose range both for DNA damage and chromosomal aberrat
ions. The level of translocations observed is comparable with the frequenci
es of dicentrics and rings.