Neutral comet assay. The comparison of several modifications

Several modifications of lysis and the same electrophoresis conditions have been applied on K562 cells in order to find optimal conditions of single c ell gel electrophoresis for manifestation of double strand breaks. The most significant difference in DNA migration between untreated K562, and treate d cells was observed when the lysis was performed at 50 degrees C. Two lysi s modifications have been choosen and applied on human lymphocytes, but nei ther of these conditions were suitable for human lymphocytes because of ext ensive DNA migration in untreated cells.