Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B-oleracea

Erwinia soft rot is a destructive disease of Brassica rapa vegetables. Reli able sources of resistance and control methods are limited, so development of highly resistant breeding lines is desirable. Protoplasts from B. rapa a nd B. oleracea genotypes selected for resistance to soft rot were fused in order to combine different sources of resistance. Twelve somatic hybrids (s ynthetic B. napus) were obtained and confirmed by morphology, nuclear DNA c ontent, and RAPD analysis. They were normal looking plants that easily set seeds following self-pollination and backcrossing to B. rapa. Assays of det ached leaves or seedlings inoculated in a mist-chamber showed that most som atic hybrids had lower disease severity ratings than the B. rapa fusion par tner and a commercial variety of B. napus. Some progeny from selfing or bac kcrossing of somatic hybrids to B. rapa showed much more resistance than ei ther fusion partner. The offspring populations of the somatic hybrids (F-1- S-1 and F-1-BC1) clearly moved to the resistant direction compared to the p arents; the percentage of resistant plants increased from 21% (average of p arents) to 36% (F-1-S-1) and 48% (F-1-BC1). These results suggest that it m ay be possible to obtain highly resistant B. rapa lines by further backcros sing and selection.