Subsite mapping of purified glucoamylase I, II, III produced by Arthrobotrys amerospora ATCC 34468

Arthrobotrys amerospora ATCC 34468 produced glucoamylase in a medium contai ning maize starch as carbon source. On native PAGE, crude glucoamylase show ed three isoenzymes which were designated as Glu I, Glu II, Glu III accordi ng to their electrophoretic mobility. These were purified by column chromat ography techniques. The energy of binding for each glucoamylase was calcula ted using Hiromi's kinetic based calculation. At subsite 1, the binding ene rgies for Glu I, II and III were found to be negative.