Systematic disruption of 456 ORFs in the yeast Saccharomyces cerevisiae

Citation
A. Lucau-danila et al., Systematic disruption of 456 ORFs in the yeast Saccharomyces cerevisiae, YEAST, 16(6), 2000, pp. 547-552
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
YEAST
ISSN journal
0749503X → ACNP
Volume
16
Issue
6
Year of publication
2000
Pages
547 - 552
Database
ISI
SICI code
0749-503X(200004)16:6<547:SDO4OI>2.0.ZU;2-I
Abstract
In the framework of the European Network for Functional Analysis (EUROFAN), five packages of 96 ORFs from chromosomes III, IV, VII, XIII, XIV and XV w ere subjected to systematic deletions in an isogenic derivative of strain S 288c, Deletions were constructed in diploid and haploid strains. Two questi onable ORFs overlapping with larger ORFs and seven TY ORFs were discarded. A total of 456 heterozygous and 385 homozygous deletant diploids were obtai ned. Sixty-nine deletions, 25 of which had never been published before, wer e lethal in haploid strains and 30 caused slow cellular growth. Copyright ( C) 2000 John Wiley & Sons, Ltd.