In the framework of the European Network for Functional Analysis (EUROFAN),
five packages of 96 ORFs from chromosomes III, IV, VII, XIII, XIV and XV w
ere subjected to systematic deletions in an isogenic derivative of strain S
288c, Deletions were constructed in diploid and haploid strains. Two questi
onable ORFs overlapping with larger ORFs and seven TY ORFs were discarded.
A total of 456 heterozygous and 385 homozygous deletant diploids were obtai
ned. Sixty-nine deletions, 25 of which had never been published before, wer
e lethal in haploid strains and 30 caused slow cellular growth. Copyright (
C) 2000 John Wiley & Sons, Ltd.