Systematic disruption of 456 ORFs in the yeast Saccharomyces cerevisiae

In the framework of the European Network for Functional Analysis (EUROFAN), five packages of 96 ORFs from chromosomes III, IV, VII, XIII, XIV and XV w ere subjected to systematic deletions in an isogenic derivative of strain S 288c, Deletions were constructed in diploid and haploid strains. Two questi onable ORFs overlapping with larger ORFs and seven TY ORFs were discarded. A total of 456 heterozygous and 385 homozygous deletant diploids were obtai ned. Sixty-nine deletions, 25 of which had never been published before, wer e lethal in haploid strains and 30 caused slow cellular growth. Copyright ( C) 2000 John Wiley & Sons, Ltd.