Crystallization and preliminary X-ray diffraction studies of phospholipaseD from Streptomyces sp.

Authors
Leiros, I Hough, E D'Arrigo, P Carrea, G Pedrocchi-Fantoni, G Secundo, F Servi, S
Citation
I. Leiros et al., Crystallization and preliminary X-ray diffraction studies of phospholipaseD from Streptomyces sp., ACT CRYST D, 56, 2000, pp. 466-468
Citations number
21
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
4
Pages
466 - 468
Database
ISI
SICI code
0907-4449(200004)56:<466:CAPXDS>2.0.ZU;2-B
Abstract
Crystals of purified phospholipase D (E.C. 3.1.4.4) from Streptomyces sp. s train PMF have been grown under two different crystallization conditions us ing vapour diffusion. Both conditions gave monoclinic crystals in space gro up P2(1). The unit-cell parameters were a = 57.28, b = 57.42, c = 68.70 Ang strom, beta = 93.17 degrees. The crystals diffract at 110 K to a resolution beyond 1.4 Angstrom using synchrotron radiation.