The effects of water extracts of CagA positive or negative Helicobacter pylori on proliferation, apoptosis and connexin formation in acetic acid-induced gastric ulcer of rats
T. Mine et al., The effects of water extracts of CagA positive or negative Helicobacter pylori on proliferation, apoptosis and connexin formation in acetic acid-induced gastric ulcer of rats, ALIM PHARM, 14, 2000, pp. 199-204
Aim: To investigate the influence of water extracts of CagA-positive or -ne
gative Helicobacter pylori on healing of chronic gastric ulcers and on conn
exin formation, proliferation and apoptosis, in acetic acid-induced chronic
gastric ulcers in rats.
Methods: Acetic acid was used to induce chronic gastric ulcers in rats, and
a water extract of H. pylori was given by mouth every day. Connexin 32 for
mation was assessed using Western blotting as previously described. The fre
quencies of proliferating cell nuclear antigen staining and of TdT-mediated
dUTP-biotin nick end labelling were examined.
Results: In untreated rats, acetic acid-induced gastric ulcers healed after
14 days and the electrophoretic band corresponding to connexin 32 appeared
4 days after ulcer induction. Treatment with a water extract of H. pylori
delayed ulcer healing, with the ulcers remaining unhealed even on the 14th
day; healing was delayed more when treatment was with an extract of CagA-po
sitive rather than CagA-negative a pylori. Connexin 32 appeared earlier whe
n treatment was with a CagA-negative rather than a CagA-positive extract, b
ut in both cases later than in the untreated control group. Proliferating c
ell nuclear antigen labelling on the fourth day was seen in 14.5% +/- 1.6%
of mucosal cells of control group, but in 35.9% +/- 1.4% and 36.5% +/- 1.4%
of mucosal cells treated with either VacA(+)CagA(-) and VacA(+)CagA(+) H.
pylori extract, respectively. Furthermore, extracts of both a pylori strain
s, especially VacA(+)Cag(+) H. pylori promoted apoptosis.
Conclusions: A water extract of H. pylori increased both proliferation and
apoptosis, which are related to excerbation and healing of ulcer as well as
appearance of connexin 32.