Immunohistochemical staining of human alpha-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

The purpose of this study was the immunohistochemical localization and dist ribution of HNP-1 in the submandibular glands of patients with oral carcino mas Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells i n the submandibular glands were stained with anti-defensin antibody. Neutro phils and capillary intimal cells were also stained Defensins (HNPS) are pe ptides that occur in neutrophils and protect against bacteria and tumor cel ls. Human alpha-defensin-1 (HNP-1) is such a peptide, possessing both antim icrobial and cytotoxic activities. The presence of HNP-1 in striated duct c ells in the submandibular glands of oval cancer patients, suggests a likely role in tumor immunity, for this peptide.