Use of integrated cell culture-PCR to evaluate the effectiveness of poliovirus inactivation by chlorine

Authors
Blackmer, F Reynolds, KA Gerba, CP Pepper, IL
Citation
F. Blackmer et al., Use of integrated cell culture-PCR to evaluate the effectiveness of poliovirus inactivation by chlorine, APPL ENVIR, 66(5), 2000, pp. 2267-2268
Citations number
11
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
66
Issue
5
Year of publication
2000
Pages
2267 - 2268
Database
ISI
SICI code
0099-2240(200005)66:5<2267:UOICCT>2.0.ZU;2-A
Abstract
Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, int egrated cell culture-PCR detected viruses for up to 8 min of exposure to th e same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to fiv e times greater than previously thought.