Fm. Plieva et al., Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media, BIOTECH LET, 22(7), 2000, pp. 551-554
Hog pancreas lipase was covalently attached to the beads of poly(vinyl alco
hol)-cryogel - a macroporous hydrogel prepared by means of freeze-thaw tech
nique. The immobilized biocatalyst thus obtained was examined in the reacti
on of enantioselective hydrolysis of the ethyl ester of N-benzylidene deriv
ative of DL-phehylalanine in the medium of acetonitrile (contained 5 vol.%
of water without any buffers). Eighty-three %-enantiomeric excess of the L-
amino acid was reached after 144 h. Virtually the same result was obtained
in the repeated use of the same immobilized biocatalyst after its 6-months-
storing in a refrigerator.