Inhibition of protein kinase C counteracts TNF alpha-induced intercellularadhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells

Authors
Defazio, G Nico, B Trojano, M Ribatti, D Giorelli, M Ricchiuti, F Martino, D Roncali, L Livrea, P
Citation
G. Defazio et al., Inhibition of protein kinase C counteracts TNF alpha-induced intercellularadhesion molecule 1 expression and fluid phase endocytosis on brain microvascular endothelial cells, BRAIN RES, 863(1-2), 2000, pp. 245-248
Citations number
17
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
00068993 → ACNP
Volume
863
Issue
1-2
Year of publication
2000
Pages
245 - 248
Database
ISI
SICI code
0006-8993(20000428)863:1-2<245:IOPKCC>2.0.ZU;2-9
Abstract
TNF alpha (100 U/ml, 24 h) upregulated intercellular adhesion molecule 1 (I CAM1) expression and fluid phase endocytosis (FPE) of horseradish peroxidas e on brain microvascular endothelial cell (BMEC) culture. The protein kinas e C (PKC) inhibitor staurosporin (0.5-10 nM) antagonized ICAM1 expression a nd FPE due to TNF alpha, whereas the protein kinase A inhibitor H89 (0.5-10 nM) did not. These findings indicate that a PKC-dependent mechanism may af fect TNFa signalling on different barrier properties of BMECs. (C) 2000 Els evier Science B.V. All rights reserved.