Cloning and identification of a novel human glioma differentiation relatedgene GDR1

In order to identify the genes associated with glioblastoma differentiation , some ESTs, expressed differentially in the control cell and the different iated human glioblastoma cell line BT-325 induced by the all-trans retinoid acid, have been isolated by the method of DDRT-PCR. Of the 46 ESTs sequenc ed, 19 are from new genes. A full-length 1 535-bp cDNA, termed gene GDR1, h as been isolated from the human cDNA library using the probe designed accor ding to one of the novel ESTs, HGBB098. The open reading frame of GDR1 gene encodes a putative protein containing 334 amino acid residues. Blast again st the current GenBank DNA and protein sequence database did not reveal sig nificant homology with any known proteins. RT-PCR shows that GDR1 mRNA leve l increased in the differentiated BT-325 cells after being treated with RA. The different expression patterns of GDR1 mRNA in human tissues have been detected through the multiple tissue Northern blot hybridization.