Cloning and identification of magnetic field-responsive genes in Daudi cells

In order to study the molecular mechanisms of ELF MF biological effects, th e differential display (DD) technology has been used to isolate differentia lly expressed cDNA fragments in Daudi cells after 50 Hz MF exposure and sha m-exposure. Using total RNA isolated from 0.8 mT 50 Hz MF exposed cells and those from sham-exposed cells, one differentially regulated transcript has been found by DD and the expression pattern of the same transcript has bee n verified by reverse-Northern and Northern analyses. DNA sequence analysis and Genbank research demonstrated that the cDNA fragment (MF-CA) is a nove l gene in human cell which is induced by ELF MF.