Spectral imaging of red blood cells in experimental anemia of Cyprinus carpio

In the present work we have studied the effect of experimental anemia induc ed at both low and optimal temperatures on erythropoiesis in Cyprinus carpi o. The results showed that hemoglobin concentration per cell was similar in both temperature conditions, however, red blood cell (RBC) concentration w as higher at the optimal temperature. Induced anemia caused an abrupt decre ase in RBC concentration, while the hemoglobin concentration per cell remai ned unchanged. Recovery, as shown by electron microscopy, was characterized by the release of differentiating young and intermediate cells to the peri pheral blood. It was revealed that with the progression of differentiation the nucleus/cytoplasm ratio decreases, the chromatin condenses and the shap e of the nucleus changes from round to elliptical. Spectral imaging reveale d an increase in the optical density of chromatin with the maturation of th e cells. The chromatin that was dispersed over the nuclear volume in the yo ung cells becomes highly ordered in the mature cells. Spectral similarity m apping revealed the formation of a novel structure of high symmetry, repres enting chromatin rearrangement during the process of cellular differentiati on. (C) 2000 Elsevier Science Inc. All rights reserved.