Structural analysis of the core region of lipopolysaccharides from Proteusmirabilis serotypes O6, O48 and O57

The structure of lipid A core region of the lipopolysaccharides (LPS) from Proteus mirabilis serotypes O6, O57 and O48 was determined using NMR, MS an d chemical analysis of the oligosaccharides, obtained by mild acid hydrolys is, alkaline deacylation, and deamination of LPS: [GRAPHICS] Incomplete substitutions are indicated by bold italic type. All sugars are present in pyranose form, alpha-Hep is the residue of L-glycero-alpha-D-man no-Hep, alpha-DD-Hep is the residue of D-glycero-alpha-D-manno-Hep, L-Ara4N is 4-amino-4-deoxy-L-arabinose, Qui4NAlaAla is the residue of 4-N-(L-alany l-L-alanyl)-4-amino-4,6-dideoxyglucose. All sugars except L-Ara4N have D-co nfiguration. beta-GalA* is partially present in the form of amide with 1,4- diaminobutane (putrescine)-HN(CH2)(4)NH2 or spermidine-HN(CH2)(3)NH(CH2)(4) NH2.