Fibroblast growth factors-5 and-9 distinctly regulate expression and function of the gap junction protein connexin43 in cultured astroglial cells from different brain regions

Astroglial cells contribute to neuronal maintenance and function in the nor mal and diseased brain. Gap junctions formed predominantly by connexin43 (c x43) provide important pathways to coordinate astroglial responses. We have previously shown that fibroblast growth factor (FGF)-2, which occurs ubiqu itously in the CNS, downregulates gap junction communication in cortical an d striatal, but not in mesencephalic astroglial cells in vitro (Reuss et al . Glia 22:19-30, 1998). Other members of the FGF family expressed in the CN S include FGF-5 and FGF-9. We show that both FGF-5 and FGF-9, like FGF-2, d ownregulate astroglial gap junctions and functional coupling. However, thei r effects are strikingly different from different brain regions, with regar d to astroglial cells. FGF-5 specifically affects mesencephalic astroglial cells without changing coupling of cortical and striatal astroglia, while F GF-9 reduces gap junctional coupling in astroglia from all three brain regi ons. Both cx43 mRNA and protein levels as well as functional coupling asses sed by dye spreading are affected. To clarify whether brain region-specific effects of FGFs on astroglial coupling are due to differential expression of FGF receptors (FGFR), we monitored expression of the four known FGFR mRN As in astroglial cultures by RT-PCR. Irrespective of their regional origin, astroglial cells express mRNAs for FGFR-8 and FGFR-3. In summary, our resu lts provide evidence for an important role of FGF-2, -5, and -9 in a distin ct, CNS region-specific regulation mechanism of astroglial gap junction com munication. The molecular basis underlying the regionally distinct responsi veness of astrocytes to different FGFs may be sought beyond distinct FGFR e xpression. (C) 2000 Wiley-Liss, Inc.