Efficient gene transfer into primary human CD8(+) T lymphocytes by MuLV-10A1 retrovirus pseudotype

Efficient and stable gene transfer into primary human T lymphocytes would g reatly improve their use for adoptive transfer to treat acquired disorders, viral diseases, and cancer. We have constructed retroviral vector pseudoty pes of amphotropic murine leukemia viruses (A-MuLV, MuLV-10Al), gibbon ape leukemia virus (GaLV), and feline endogenous virus (RD114) containing the e nhanced green fluorescent protein (GFP) as a marker gene. Transduction of p rimary human CD8(+) T lymphocytes by the different CFP-retrovirus pseudotyp es revealed the superiority of MuLV-10Al in comparison with A-MuLV, GaLV, a nd RD114, respectively. The superior transduction efficacy of CD8(+) T cell s by MuLV-10Al correlates with a longer half-life of this pseudotype in com parison with A-MuLV and, as shown by interference analysis with the human T cell line HUT78, by the utilization of both the A-MuLV receptor (Pit2) and the GaLV receptor (Pit1) for cell entry.