OXYGEN INCREASES THE STEADY-STATE LEVEL OF NITRATE IN DENITRIFYING CELLS OF PARACOCCUS-DENITRIFICANS

Authors
KUCERA I KAPLAN P ZEMAN A
Citation
I. Kucera et al., OXYGEN INCREASES THE STEADY-STATE LEVEL OF NITRATE IN DENITRIFYING CELLS OF PARACOCCUS-DENITRIFICANS, FEMS microbiology letters, 145(2), 1996, pp. 163-166
Citations number
12
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
145
Issue
2
Year of publication
1996
Pages
163 - 166
Database
ISI
SICI code
0378-1097(1996)145:2<163:OITSLO>2.0.ZU;2-3
Abstract
The levels of nitrate in denitrifying cells of Paracoccus denitrifican s were determined by centrifugation through silicone oil into phosphor ic acid and ion-exchange HPLC analysis of the cell lysates, using [C-1 4]sucrose to correct for the trapped external medium. Introduction of oxygen brought about a significant upward shift in the intracellular n itrate concentration. This result calls into question the current thin king that oxygen blocks nitrate respiration primarily due to the inhib ition of nitrate transport into the cell.