C. Choi et al., Loss of heterozygosity at chromosome segments 8p22 and 8p11.2-21.1 in transitional-cell carcinoma of the urinary bladder, INT J CANC, 86(4), 2000, pp. 501-511
To identify the putative tumor-suppressor gene (TSG) involved in transition
al-cell carcinoma (TCC) of the urinary bladder, we undertook an allelotypin
g analysis in 48 cases of TCC. Relatively high percentages of allelic loss
were found in 2p (5 of 23, 21.7%), 8p (9 of 2i, 42.9%), 9p (4 of 20, 20.0%)
, 12q (6 of 28, 21.4%), 15q (1 of 5, 20%; 4 of 20, 20%), 17p (7 of 26, 26.9
%) and 22q (6 of 23, 26.1%). On the basis of these results, fine-deletion m
apping was performed on chromosome 8 in 52 cases by PCR of 15 microsatellit
e markers. Two distinct regions of common deletion were found. A 10 cM telo
meric region was located to 8p22, defined by D8S511 and D8S258. A 17 cM cen
tromeric region was located to 8p 11.2-21.1,flanked by D8S298 and D8S535. T
he distance between the telomeric and the centromeric regions of common del
etion was 3 cM. Loss of heterozygosity of 8p22 was frequently observed in t
umors of high grade or advanced stage. Int. J. Cancer 86: 501-505, 2000. (C
) 2000 Wiley-Liss, Inc.