Gene expression profiling of advanced lung cancer

Lung cancer is a highly aggressive neoplasm with 85% mortality. To identify new tumor-associated genes, we compared the expression profile of a primar y metastasizing adenocarcinoma with normal airway epithelial cells. Two cDN A libraries of up- and downregulated genes were generated, comprising 253 a nd 299 clones, respectively. The sequence analysis revealed 205 different k nown genes and 314 cDNA fragments of unknown functions. Northern-blot analy sis of 167 clones confirmed differential expression in 58%, and indicated a similar expression pattern in additional lung-cancer cell lines for select ed clones, strengthening the value of this model for the identification of new candidate genes in lung carcinogenesis. Int. J. Cancer 86:512-517, 2000 . (C) 2000 Wiley-Liss, Inc.