A. Van Den Berg et al., Frequent lack of translation of antigen presentation-associated molecules MHC class I, CD1a and B-2-microglobulin in Reed-Sternberg cells, INT J CANC, 86(4), 2000, pp. 548-552
Epstein-Barr virus (EBV) is present in Reed-Sternberg (RS) cells of a subst
antial proportion of Hodgkin's lymphoma cases. Most EBV-positive cases are
also MHC class I-positive, whereas the majority of EBV-negative cases lack
detectable levels of MHC class I expression. Application of the SAGE techni
que has led to the identification of tags corresponding to MHC class I and
beta(2)-microglobulin genes in the EBV- and MHC class I-negative L428 Hodgk
in's cell line. Further expression studies indicated that single RS cells t
hat do not express HLA class I also lack beta(2)-microglobulins but frequen
tly contain mRNA coding for these proteins. Another tag was identified corr
esponding to CD1a, a thymocyte and Langerhans cell antigen structurally rel
ated to the MHC class I genes. CD1a expression studies revealed mRNA in all
cell lines and in several of the single cells, whereas immunostaining show
ed a cytoplasmic signal in only 2 of the 4 cell lines and in none of the Ho
dgkin's lymphoma tissue samples. In conclusion, RS cells frequently lack MH
C class I, beta(2)-microglobulin and CD1a protein expression but contain mR
NA coding for these proteins in some of the RS cells, suggesting a common m
echanism affecting the translation of these antigen presentation-associated
molecules. Int. J. Cancer 86:548-552, 2000. (C) 2000 Wiley-Liss, Inc.