Identification of genes responsible for improved cryoresistance in fermenting yeast cells

Using repetitive freezing and thawing, different mutant industrial Saccharo myces cerevisiae strains with increased freeze resistance have been isolate d. To get a better insight in the mechanisms responsible for this elevated resistance and to give us the opportunity to modify other strains so that t hey become more suitable for use in frozen dough preparations, we applied t he microarray technology in order to identify genes that are differentially expressed in a freeze-resistant mutant when compared to a freeze-sensitive industrial yeast strain. (C) 2000 Elsevier Science B.V. All rights reserve d.