Cf. Snook et al., Use of a crystallization robot to set up sitting-drop vapor-diffusion crystallization and in situ crystallization screens, J APPL CRYS, 33, 2000, pp. 344-349
A commercial crystallization robot has been modified for use in setting up
sitting-drop vapor-diffusion crystallization experiments, and for setting u
p protein crystallization screens in situ. The primary aim of this effort i
s the automated screening of crystallization of integral membrane proteins
in detergent-containing solutions. However, the results of this work are of
general utility to robotic liquid-handling systems. Sources of error that
can prevent the accurate dispensing and mixing of solutions have been ident
ified, and include local environmental, machine-specific and solution condi
tions. Solutions to each of these problems have been developed and implemen
ted. (C) 2000 Internalional Union of Crystallography Printed in Great Brita
in - all rights reserved.