Cyclin D1 binds and regulates the activity of cyclin-dependent kinases (CDK
s) 4 and 6. Phosphorylation of the retinoblastoma protein by cyclin D1-CDK4
/6 complexes during the G(1) phase of the cell cycle promotes entry into S
phase. Cyclin D1 protein is ubiquitinated and degraded by the 26 S proteaso
me, Previous studies have demonstrated that cyclin D1 ubiquitination is dep
endent on its phosphorylation by glycogen synthase kinase 3 beta (GSK-3 bet
a) on threonine 286 and that this phosphorylation event is greatly enhanced
by binding to CDK4 (Diehl, J. A., Cheng, M. G., Roussel, M. F., and Sherr,
C. J. (1998) Genes Dev. 12, 3499-3511). We now report an additional pathwa
y for the ubiquitination of free cyclin D1 (unbound to CDKs). We show that,
when unbound to CDK4, a cyclin D1-T286A mutant is ubiquitinated. Further,
we show that a mutant of cyclin D1 that cannot bind to CDK4 (cyclin D1-KE)
is also ubiquitinated in vivo. Our results demonstrate that free cyclin D1
is ubiquitinated independently of its phosphorylation on threonine 286 by G
SK-3 beta, suggesting that, as has been shown for cyclin E, distinct pathwa
ys of ubiquitination lead to the degradation of free and CDK-bound cyclin D
1. The pathway responsible for ubiquitination of free cyclin D1 may be impo
rtant in limiting the effects of cyclin DI overexpression in a variety of c
ancers.