Y. Yanagida et al., Electrically stimulated induction of hsp70 gene expression in mouse astroglia and fibroblast cells, J BIOTECH, 79(1), 2000, pp. 53-61
Mouse astroglial cells, which were cultured on an electrode, were found res
ponsive to an electric stimulation of sine wave potential in enhancing hsp7
0 mRNA resulting from an activation of hsp70 gene expression. On the basis
of this finding, electrically responsive cells were established by transfec
ting mouse 3T3-L1 cells with a constructed plasmid encoding hsp70 promoter
and the firefly luciferase gene. A stable cell line has been established th
rough selection of heat-stimulated luciferase expression. A 1-h electric st
imulation of the cells resulted in activation of luciferase expression, whi
ch was confirmed to produce an increase in light emission. The sequential p
attern of the electrically stimulated expression of luciferase was found di
fferent from that of the heat stimulation. Furthermore, the promoter was ac
tivated depending on the potential and duration of the stimulation applied.
Consequently, the electric stimulation has proven effective on activating
hspP70 promoter. This cell line is feasible in expressing the gene of inter
est by electrical stimulation, which lead us to construct environment respo
nsive cells in general. (C) 2000 Elsevier Science B.V. All rights reserved.