Tumor cells in vivo often exist in an ischemic microenvironment that would
compromise the growth of normal cells. To minimize intracellular acidificat
ion under these conditions, these cells are thought to upregulate H+ transp
ort mechanisms and/or slow the rate at which metabolic processes generate i
ntracellular protons. Proton extrusion has been compared under identical co
nditions in two closely related human breast cell lines: nonmalignant but i
mmortalized HMT-3522/S1 and malignant HMT-3522/T4-2 cells derived from them
. Only the latter were capable of tumor formation in host animals or long-t
erm growth in a low-pH medium designed to mimic conditions in many solid tu
mors. However, detailed study of the dynamics of proton extrusion in the tw
o cell lines revealed no significant differences. Thus, even though the abi
lity to upregulate proton extrusion in a low pH environment (pH(e)) may be
important for cell survival in a tumor, this ability is not acquired along
with the capacity to form solid tumors and is not unique to the transformed
cell. This conclusion was based on fluorescence measurements of intracellu
lar pH (pH(i)) on cells that were plated on extracellular matrix, allowing
them to remain adherent to proteins to which they had become attached 24 to
48 h earlier. Proton translocation under conditions of low pH(e) was obser
ved by monitoring pH(i) after exposing cells to an acute acidification of t
he surrounding medium. Proton translocation at normal pH(e) was measured by
monitoring the recovery after introduction of an intracellular proton load
by treatment with ammonium chloride. Even in the presence of inhibitors of
the three major mechanisms of proton translocation (sodium-proton antiport
, bicarbonate transport, and proton-lactate symport) together with acidific
ation of their medium, cells showed only about 0.4 units of reduction in pH
(i). This was attributed to a slowing of metabolic proton generation becaus
e the inhibitors were shown to be effective when the same cells were given
an intracellular acidification. J. Cell. Physiol. 183:373-380, 2000. (C) 20
00 Wiley-Liss, Inc.