Fast chiral separations using sulfated beta-cyclodextrin and short-end injection in capillary electrophoresis

The general applicability of sulfated beta-cyclodextrin as chiral selector and short-end injection in capillary electrophoresis (CE) as a powerful scr eening tool for fast and efficient chiral separation of Ormeloxifene enanti omers and racemic Ormeloxifene analogues is demonstrated. Using the short-e nd injection procedure, all of the 16 racemic compounds studied were succes sfully separated with high efficiencies and with analysis times of less tha n 1.2 min. Furthermore, long-end injections of eight analogues named C1-C8 afforded separations with extremely high efficiencies. A statistical evalua tion of the resolution values obtained in short-end and long-end injections of compounds C1-C8 showed that the sensitivity of the CE method towards st ructural changes in the studied molecules is intact when the chiral analysi s is performed with short-end injection compared to conventional long-end i njection. (C) 2000 Elsevier Science BN. All rights reserved.