C. Redies et al., Morphologic fate of diencephalic prosomeres and their subdivisions revealed by mapping cadherin expression, J COMP NEUR, 421(4), 2000, pp. 481-514
The expression of four cadherins (cadherin-6B, cadherin-7, R-cadherin, and
N-cadherin) was mapped in the diencephalon of chicken embryos at 11 days an
d 15 days of incubation and was compared with Nissl stains and radial glial
topology. Results showed that each cadherin is expressed in a restricted m
anner by a different set of embryonic divisions, brain nuclei, and their su
bregions. An analysis of the segmental organization based on the prosomeric
model indicated that, in the mature diencephalon, each prosomere persists
and forms a coherent domain of gray matter extending across the entire tran
sverse dimension of the neural tube, from the ventricular surface to the pi
al surface. Moreover, the results suggest the presence of a novel set of se
condary subdivisions for the dorsal thalamus (dorsal, intermediate, and ven
tral tiers and anteroventral subregion). They also confirm the presence of
secondary subdivisions in the pretectum (commissural, juxtacommissural, and
precommissural). At most of the borders between the prosomeres and their s
econdary subdivisions, changes in radial glial fiber density were observed.
The diencephalic brain nuclei that derive from each of the subdivisions we
re determined. In addition, a number of previously less well-characterized
gray matter regions of the diencephalon were defined in more detail based o
n the mapping of cadherin expression. The results demonstrate in detail how
the divisions of the early embryonic diencephalon persist and transform in
to mature gray matter architecture during brain morphogenesis, and they sup
port the hypothesis that cadherins play a role in this process by providing
a framework of potentially adhesive specificities. J. Comp. Neurol. 421:48
1-514, 2000. (C) 2000 Wiley-Liss, Inc.