Interaction between NiCl2 and nucleobases, nucleosides and nucleotides

The interaction between NiCl2 and nucleobases, nucleosides and nucleotides has been studied by UV-Vis difference spectrophotometry, graphite furnace a tomic absorption spectrophotometry, IR spectroscopy and high pressure liqui d chromatography using the technique of continuous variation. The proposed structures of the complexes Formed were optimised and their electronic and vibrational spectra generated using the molecular modelling program HyperCh em 5. Ni2+ reacts with guanine, 2'-dGMP, CMP, adenine and AMP to form 1:1 c omplexes Ni(Guanine)(H2O)(5), Ni(2'-dGMP)(H2O)(5) Ni(GMP)(H2O)(5), Ni(Adeni ne) (H2O)(5) and Ni(AMP)(H2O)(5) respectively. Tn these complexes, Ni2+ is believed to be bonded to the N7 atom of adenine and guanine. (C) 2000 Elsev ier Science Inc. All rights reserved.