Two novel secoergosterols from the fungus Tylopilus plumbeoviolaceus

Two novel secoergosterols, 3 beta-hydroxy-8 alpha,9 alpha-oxido-8,9-secoerg osta-7,9(11),22-triene (tylopiol A) (1) and 3 beta-hydroxy-8 alpha,9 alpha- oxido-8,9-secoergosta-7,22dien-12-one (tylopiol B) (2), were isolated from the fresh fruit bodies of Tylopilus plumbeoviolaceus, along with three know n compounds, ergosta-7,22-dien-3 beta-ol, uridine, and allitol. Their struc tures were elucidated by NMR techniques, including H-1 NMR, C-13 NMR, HMQC, HMBC, and MS. The structure and stereochemistry of compound 1 were demonst rated by X-ray crystallography.